- PII
- S19982860S0132342325040158-1
- DOI
- 10.7868/S1998286025040158
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 51 / Issue number 4
- Pages
- 715-723
- Abstract
- Obtaining a fraction of double-stranded RNA is an integral part of any RNA interference research whether it aimed at solving fundamental or applied problems. The production of dsRNA in bacterial culture is a common technique due to its comparative cheapness and scaling-up opportunities. In this article, we propose a new method for fast and effective isolation of dsRNA from bacterial culture, as an alternative to classical phenol-chloroform extraction. In our method, phenol is replaced with less toxic methanol, and the total RNA thus isolated from bacteria contains up to 25% of the target molecule lacking the DNA contamination, which enables its usage in certain further applications without additional cleanup steps. The application of this methodology will be justified in laboratories engaged in either fundamental or applied research on RNA interference. However, scaling the technology for agricultural use may require adjustments to the protocol described in this work.
- Keywords
- двуцепочечная РНК выделение РНК наработка дцРНК в бактериях РНК-интерференция спрей-индуцированный сайленсинг генов
- Date of publication
- 18.12.2024
- Year of publication
- 2024
- Number of purchasers
- 0
- Views
- 13
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