- PII
- S19982860S0132342325030145-1
- DOI
- 10.7868/S1998286025030145
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 51 / Issue number 3
- Pages
- 523-530
- Abstract
- Here we investigate the effect of the C-terminal region on the solubility of TLR4 and TLR10 TIR domains upon the heterologous expression in Escherichia coli. We demonstrated that the absence of this region significantly enhances the yields of globular domains in a soluble form. These findings complement the protocols, previously established to synthesize the TLR TIR domains for the structural studies by various techniques, including by NMR spectroscopy.
- Keywords
- TIR-домен экспрессия растворимость рекомбинантный белок TLR Toll-подобный рецептор мембранный белок структура
- Date of publication
- 07.12.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 5
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