- PII
- S19982860S0132342325030062-1
- DOI
- 10.7868/S1998286025030062
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 51 / Issue number 3
- Pages
- 432-443
- Abstract
- Biochips with protein and oligonucleotide probes are used to analyze protein and nucleic acid samples. The key challenges of the technology are the selection of substrate materials and surface functionalization. Polybutylene terephthalate substrates were modified by coating them with photoactive polymers: poly(ethylene-co-propylene-co-5-methylene-2-norbornene), acetylcellulose, polyvinyl acetate and polyvinyl butyral. The coatings were applied by centrifugation and dried. The effect of the coating on the biochip characteristics was investigated. A matrix of hydrophilic cells made of brush polymers with epoxy groups for immobilization of DNA probes and human immunoglobulins was prepared by photoinitiated radical polymerization. The functionality of probes was investigated by hybridization analysis and reaction with specific antibodies. The binding efficiency of probes to molecular targets was evaluated on biochips with different coatings. Cells on substrates coated with polyvinyl butyral and poly(ethylene-co-propylene-co-5-methylene-2-norbornene) showed the best binding efficiency and weak adsorption of targets, providing high contrast fluorescence images after probe binding. Biochips on such substrates are promising for lab-on-a-chip microanalysis technology.
- Keywords
- полимерные поверхности биочипы щеточные полимеры иммобилизация олигонуклеотидных зондов иммобилизация белков гибридизационный анализ флуоресцентный иммуноанализ
- Date of publication
- 07.12.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 5
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