Development of a Bacterial Expression System for Producing N/C-Labeled Neuroglobin and Cytochrome C

An effective system for producing isotopically labeled heme-containing proteins of human neuroglobin and cytochrome c has been developed. The corresponding producer strains have been obtained, and optimal cultivation conditions have been selected: temperature and duration of incubation, composition of media, and concentration of expression inducer. Using far- and near-UV-vis CD spectroscopy, it was shown that the secondary structure composition of N-neuroglobin is close to the calculated one, and the orientation of the heme in the molecules is predominantly canonical. According to two-dimensional H/N-HSQC NMR spectra of human neuroglobin and cytochrome c, the proteins are folded into a native conformation with a predominance of the -helical structure. The resulting production system can be used to produce highly purified preparations of totally N/C-labeled proteins for structural-dynamic studies using modern high-resolution NMR spectroscopy methods.

Keywords

нейроглобин цитохром с ЯМР гемсодержащие белки рекомбинантные белки

Date of publication

01.05.2025

Year of publication

2025

Number of purchasers

Views

References

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GOST	Bershatsky Y., Bocharov E., Britikov V., Britikova E., Chertkova R., Dolgikh D., Ignatova A., Khodnenko A., Kirpichnikov M., Semenova M., Smirnova O. Development of a Bacterial Expression System for Producing N/C-Labeled Neuroglobin and Cytochrome C // Russian Journal of Bioorganic Chemistry. – 2025. – V. 51. – Issue number 5 C. 899-910 . URL: https://bioorgchemras.ru/s19982860s0132342325050159-1/?version_id=120722. DOI: 10.7868/S1998286025050159
MLA	Bershatsky, Y. V, Bocharov, E. V, Britikov, V. V, Britikova, E. V, Chertkova, R. V, Dolgikh, D. A, Ignatova, A. A, Khodnenko, A. P, Kirpichnikov, M. P, Semenova, M. A, Smirnova, O. M "Development of a Bacterial Expression System for Producing N/C-Labeled Neuroglobin and Cytochrome C." Russian Journal of Bioorganic Chemistry. 51.5 (2025).:899-910. DOI: 10.7868/S1998286025050159
APA	Bershatsky Y., Bocharov E., Britikov V., Britikova E., Chertkova R., Dolgikh D., Ignatova A., Khodnenko A., Kirpichnikov M., Semenova M., Smirnova O. (2025). Development of a Bacterial Expression System for Producing N/C-Labeled Neuroglobin and Cytochrome C. Russian Journal of Bioorganic Chemistry. vol. 51, no. 5, pp.899-910 DOI: 10.7868/S1998286025050159

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Development of a Bacterial Expression System for Producing N/C-Labeled Neuroglobin and Cytochrome C

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RAS BiologyБиоорганическая химия Russian Journal of Bioorganic Chemistry

Development of a Bacterial Expression System for Producing N/C-Labeled Neuroglobin and Cytochrome C

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