- PII
- S19982860S0132342325050141-1
- DOI
- 10.7868/S1998286025050141
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 51 / Issue number 5
- Pages
- 892-898
- Abstract
- Development of an orthotopic intracranial model based on a human glioblastoma cell culture in immunodeficient mice is an important task both for studying the invasiveness and aggressiveness of tumor cell behavior, and for creating a reliable model to evaluate the efficacy of new drugs for glioblastoma therapy. In this study, a comparative analysis was conducted using a glioblastoma cell line (U87MG) and primary glioblastoma cells obtained from a patient (culture 022), following subcutaneous and orthotopic intracranial xenotransplantation into NSG immunodeficient mice. It was shown that in both groups of animals with orthotopic xenografts, the tumors grew both deep into the brain tissue and along the brain surface, while in the case of the primary culture 022, growth toward the ventricles was also observed. The non-cell-line-derived (primary) cells exhibited an epithelioid morphology, whereas U87MG cells showed a more sarcomatoid appearance. The U87MG cell line was tumorigenic in both locations. However, the primary culture 022 formed tumors only following intracranial, but not subcutaneous, xenotransplantation, indicating the neuro-specificity of this model. Therefore, it may serve as a more relevant glioblastoma model compared to the U87MG cell line-based model.
- Keywords
- глиобластома интракраниальный ксенотрансплантат туморогенность U87MG
- Date of publication
- 01.05.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 7
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