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RAS BiologyБиоорганическая химия Russian Journal of Bioorganic Chemistry

  • ISSN (Print) 0132-3423
  • ISSN (Online) 1998-2860

Molecular Probes for Visualization of Nicotinic Acetylcholine Receptors Based on Snake Three-Finger Toxins and a Red Fluorescent Protein

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PII
S19982860S0132342325050107-1
DOI
10.7868/S1998286025050107
Publication type
Article
Status
Published
Authors
A.I. Kuzmenkov
  • Affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
I.S. Chudetsky
  • Affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
D.S. Kudryavtsev
  • Affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
I.E. Kasheverov
  • Affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
V.I. Tsetlin
  • Affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
A.A. Vassilevski
  • Affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Volume/ Edition
Volume 51 / Issue number 5
Pages
853-862
Abstract
The visualization of macromolecular complexes is an important task in modern bioorganic chemistry, which can be addressed using various methodological approaches. The most widely used techniques involve radioactive- and fluorescent-labeled ligands. In this study, molecular probes were developed in the form of hybrid constructs combining one of three snake toxins (α-bungarotoxin, α-cobratoxin, or neurotoxin NT-II) with the red fluorescent protein mKate2. These chimeric proteins were produced in a bacterial expression system and purified via gel filtration. Using competitive radioligand binding assay with radiolabeled α-bungarotoxin, it was demonstrated that the obtained probes exhibit high affinity for the nicotinic acetylcholine receptor of the electric organ from T, with half-maximal inhibitory concentration values in the nanomolar range. The fluorescent probes were successfully employed for the visualization of acetylcholine receptors on the surface of SH-SYSY cells.
Keywords
ионный канал ацетилхолиновый рецептор холинорецептор лиганд нейротоксин радиолигандный анализ флуоресцентная микроскопия
Date of publication
01.05.2025
Year of publication
2025
Number of purchasers
0
Views
4

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