- PII
- S19982860S0132342325040048-1
- DOI
- 10.7868/S1998286025040048
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 51 / Issue number 4
- Pages
- 599-606
- Abstract
- The task of three-dimensional (3D) reconstruction of tissues at the cellular and subcellular level is an integral part of modern biomedical research. Developing 3D reconstruction methods based on atomic force and optical microscopy images enables a more detailed understanding of the morphology and structure of biological specimens at the nanoscale. In the present study, we integrated dedicated software into the workflow of optically probed nanotomography (OPNT) to optimize and standardize the volumetric reconstruction process and demonstrated the capabilities of the enhanced method by reconstructing a fragment of an astrocyte in 3D. The incorporation of 3D Slicer into the OPNT protocol provides a reliable, high-precision platform for the three-dimensional reconstruction of complex biological structures. The proposed approach has practical utility for specialized tasks – such as evaluating the synaptic environment of individual neurons – as well as for a wide range of investigations in biomedical research and materials science.
- Keywords
- 3D-реконструкция атомно-силовая микроскопия оптическо-зондовая нанотомография 3D Slicer астроцит биомедицинские исследования
- Date of publication
- 14.12.2024
- Year of publication
- 2024
- Number of purchasers
- 0
- Views
- 10
References
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