- PII
- S19982860S0132342325030113-1
- DOI
- 10.7868/S1998286025030113
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 51 / Issue number 3
- Pages
- 486-495
- Abstract
- Here, we studied the regulation of pou5f3 family transcripts stability by association with Ybxl, a protein of ribonucleoprotein complexes. It is known that the clawed frog Xenopus laevis has three genes belonging to the POU5 family: pou5f3.1/oct91, pou5f3.2/oct25, and pou5f3.3/oct60. The Pou5f3 family factors are orthologues of the mammalian embryonic stem cell OCT4 pluripotency factor. However, the expression patterns of these genes differ over time. Pou5f3.3/oct60 transcripts are stored in oocytes, are present in large quantities in fertilized eggs, and then degrade only after fertilization. Pou5f3.2/oct25 transcripts are also present in the zygote, but their numbers increase even more during the development process. Finally, pou5f3.1/oct91 transcription begins only after the activation of the embryo genome at the middle blastula stage. In the present work, we revealed a much higher specificity of the Ybx1 factor to form a complex with the maternal mRNA of the pou5f3.3/oct60 gene compared to zygotic mRNAs of the pou5f3.1/oct91 and pou5f3.2/oct25 genes. Since Ybx1 is a protein that, on the one hand, is involved in interaction with cytoskeletal proteins, and, on the other hand, binds and stabilizes pluripotency genes mRNA, it can play a linking role in between the degradation of these maternal transcripts and cytoskeletal rearrangements during the onset of morphogenetic cell movements in the process of formation of germ layers.
- Keywords
- эмбриогенез плюрипотентность дифференцировка транскрипционные факторы POU5 pou5f3.3 Ybx1 мРНК
- Date of publication
- 07.12.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 6
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