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RAS BiologyБиоорганическая химия Russian Journal of Bioorganic Chemistry

  • ISSN (Print) 0132-3423
  • ISSN (Online) 1998-2860

A Method for Increasing the Efficiency of Selection of Aptamers to Cellular Receptors

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PII
S19982860S0132342325030081-1
DOI
10.7868/S1998286025030081
Publication type
Article
Status
Published
Authors
V. E. Kuznetsova
  • Affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
T. D. Lebedev
  • Affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
V. E. Shershov
  • Affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
G. F. Shtylev
  • Affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
I. Yu. Shishkin
  • Affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
R. A. Miftahov
  • Affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
V. I. Butvilovskaya
  • Affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
I. V. Grechishnikova
  • Affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
O. A. Zasedateleva
  • Affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
A. V. Chudinov
  • Affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Volume/ Edition
Volume 51 / Issue number 3
Pages
451-460
Abstract
A method has been proposed to increase the efficiency of selection of aptamers to cellular receptors by the cell-Selex method, in particular to the receptor tyrosine kinase c-KIT. The use of Tween 20 in buffer solutions in concentrations not exceeding 0.01%, as well as trypsinolysis of surface proteins at the stage of elution of the combinatorial library of oligonucleotides bound to the cell surface, led to an increase in the specificity of aptamers and a decrease in nonspecific sorption according to the results of fluorescence microscopy, thermofluorimetric analysis and high-precision sequencing.
Keywords
аптамер cell-SELEX трипсин реакция удлинения праймера c-KIT Tween 20
Date of publication
07.12.2025
Year of publication
2025
Number of purchasers
0
Views
5

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