- PII
- S0132342325010131-1
- DOI
- 10.31857/S0132342325010131
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 51 / Issue number 1
- Pages
- 144-152
- Abstract
- We propose a new fluorogen-activating protein nano-frFAST with only 98 amino acids based on a combination of the previously created fluorogen-activating proteins nanoFAST and frFAST. A series of fluorogens with an increased system of conjugated bonds, which are potentially capable of binding to this protein, were synthesized. Based on the results of the study, a promising fluorogen which is (Z)-5-((E)-3-(4-hydroxy-2,5-dimethoxyphenyl)allylidene)-2-thioxothiazolidin-4-one (HPAR-DOM) was identified. We demonstrate that the nano-frFAST–HPAR-DOM complex can be used as a genetically encoded far-red fluorescent label for staining individual structures of living cells.
- Keywords
- арилиден-азолоны арилаллилиден-азолоны флуороген-активирующие белки флуорогены флуоресцентное мечение
- Date of publication
- 09.11.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 45
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