- PII
- S0132342325010064-1
- DOI
- 10.31857/S0132342325010064
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 51 / Issue number 1
- Pages
- 63-71
- Abstract
- Epigenetic histone modifications play a key role in the differentiation of stem cells into various cell types. The ability of induced pluripotent stem cells (iPSCs) to differentiate is assessed using the embryoid body formation method, which is widely used and prevalent in iPSC research. In this study, we utilized a stable line of iPSCs with a genetically encoded sensor MPP8-Green to visualize the histone modification H3K9me3 during embryoid body formation. We identified two groups of cells based on the distribution of H3K9me3 in the formed embryoid bodies, using the MPP8-Green sensor. This study demonstrates that the MPP8-Green sensor can be used to track the dynamics of H3K9me3 during spontaneous differentiation and embryoid body formation. Using the sensor, we identified two groups of cells with different distributions of H3K9me3 and showed the potential application of such genetically encoded tools to reveal differences in patterns of epigenetic modifications during the spontaneous differentiation of iPSCs.
- Keywords
- иПСК эмбриоидные тельца гистоны метилирование эпигенетическая регуляция флуоресцентная микроскопия
- Date of publication
- 09.11.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 54
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