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RAS BiologyБиоорганическая химия Russian Journal of Bioorganic Chemistry

  • ISSN (Print) 0132-3423
  • ISSN (Online) 1998-2860

Influence of the Cultivation Conditions of Glioblastoma Cells on the Expression of Transcription Factors Genes

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PII
S19982860S0132342325050181-1
DOI
10.7868/S1998286025050181
Publication type
Article
Status
Published
Authors
A. I. Rezekina
  • Affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
D. V. Mazur
  • Affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
M. I. Shakhparonov
  • Affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
N. V. Antipova
  • Affiliation:
    Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
    National Research University "Higher School of Economics"
Volume/ Edition
Volume 51 / Issue number 5
Pages
931-939
Abstract
Cellular heterogeneity is a feature of glioblastoma, a malignant and aggressive brain tumor, and one of the reasons for the ineffectiveness of standard treatment methods, probably arising from the differentiation of tumor stem cells. The causes of increased adaptive abilities and resistance of glioblastoma cells to drugs and stress arise at the level of molecular processes. For this reason, there were evaluated changes in the expression of the genes of transcription factors and , which are involved in the regulation of the cell cycle and differentiation, using real-time PCR in response to changes in cultivating conditions in this work. As a result, it was shown that primary cultures in an environment with fetal bovine serum acquire morphology and gene expression similar to cell lines. In addition, it was shown for the first time that all cell lines and primary cultures of glioblastoma differ in the expression profiles of the studied genes, however, in response to changes in cultivating conditions, all of them demonstrate a multiple increase in MYCN expression, as well as the opposite response of and , indicating a possible role of these genes in glioblastoma resistance to stress. The obtained data should be taken into account while selecting individual treatment for patients, as well as when developing therapeutic agents and further investigating molecular processes in glioblastoma cells.
Keywords
глиобластома MYCN MYCC PHOX2A PHOX2B
Date of publication
01.05.2025
Year of publication
2025
Number of purchasers
0
Views
2

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