- PII
- S19982860S0132342325050162-1
- DOI
- 10.7868/S1998286025050162
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 51 / Issue number 5
- Pages
- 911-919
- Abstract
- The properties of the voltage-gated potassium Kv1.1 channel, formed in Neuro-2a cells from dimers of the human α-subunits Kv1.1 linked by the Lys-Leu dipeptide and fused at the N-terminus with the mKate2 fluorescent protein (mKate2-(Kv1.1)), were studied. It was found that the linking of α-subunits Kv1.1 into a dimer did not affect the membrane expression of the channel and the features of its cellular distribution compared with monomers mKate2-Kv1.1. No differences were found between channels based on mKate2-(Kv1.1) dimers and mKate2-Kv1.1 monomers in the half-activation potential, channel activation constants, the magnitude and nature of potassium ion currents. The data obtained suggest the possibility of creating bioengineered protein structures by linking two different α-subunits in a similar way, which would form fluorescent heterotetrameric voltage-gated potassium channels with an α-subunit stoichiometry of 2 : 2 in mammalian cells.
- Keywords
- калиевые каналы конфокальная микроскопия петиидные блокаторы потенциал-зависимые электродвизиология
- Date of publication
- 01.05.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 5
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