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RAS BiologyБиоорганическая химия Russian Journal of Bioorganic Chemistry

  • ISSN (Print) 0132-3423
  • ISSN (Online) 1998-2860

Cytoskeletal Regulator Zyxin Stimulates Translocations of YAP into Embryo Cell Nuclei

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PII
S19982860S0132342325050114-1
DOI
10.7868/S1998286025050114
Publication type
Article
Status
Published
Authors
E.A. Parshina
  • Affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
E.E. Orlov
  • Affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
E.E. Voronezhskaya
  • Affiliation: Koltzov Institute of Developmental Biology of the Russian Academy of Sciences
N.Yu. Martynova
  • Affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
A.G. Zaraisky
  • Affiliation:
    Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
    Koltzov Institute of Developmental Biology of the Russian Academy of Sciences
    Pirogov Russian National Research Medical University
Volume/ Edition
Volume 51 / Issue number 5
Pages
863-872
Abstract
There is limited data in the scientific literature regarding the relationship between mechanotransduction associated with changes in Zyxin levels and biochemical signaling of the transcription factor YAP. Research in this area suggests that stress-induced reorganization of the actin cytoskeleton mediated by Zyxin may play a key role in YAP's mechanotransduction. However, the results of these studies do not provide clear outcomes concerning the effect of Zyxin on YAP distribution between the nucleus and the cytoplasm and its activity regulation. Here, we investigated the effects of Zyxin on the nuclear translocation of the Hippo-signaling pathway effector, YAP, in the early embryo of the clawed frog . Analysis of the nuclear-cytoplasmic distribution of YAP by immunoblotting and immunohistochemical staining, combined with the suppression of translation of endogenous Zyxin mRNA by morpholino antisense oligonucleotides and overexpression of synthetic Zyxin mRNA, revealed a stimulatory effect of Zyxin on the nuclear translocation of YAP in gastrula-stage embryos, but not in blastula and neurula stages. A similar conclusion was reached by analyzing the effect of the same changes in Zyxin concentration on the expression of a YAP-dependent luciferase reporter. Based on the results of our study, and taking into account the known role of Zyxin as one of the mechanotransducers, it can be assumed that this protein is involved in the mechano-dependent regulation of the Hippo signaling pathway in embryonic development at the gastrulation stage.
Keywords
морфогенез зиксин YAP механотрансдукция Xenopus laevis
Date of publication
01.05.2025
Year of publication
2025
Number of purchasers
0
Views
5

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